Enzyme Inhibitors An enzyme's activity can be reduced or stopped, temporarily, by a reversible inhibitor There are?two types?of reversible inhibitors: Competitive?inhibitors have a?similar shape?to...
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Vmax & the Michaelis-Menten Constant Substrate concentration?affects the?rate of catalysis?in an enzyme-substrate reaction When the substrate concentration is?fixed?(and enzyme concentration is...
Rate: Inhibitor Concentration There are?two types?of inhibitors: Competitive?inhibitors have a?similar shape?to that of the?substrate?molecules and therefore?compete?with the substrate for the acti...
Rate: Enzyme Concentration Enzyme concentration affects the rate of reaction The?higher?the?enzyme concentration?in a reaction mixture, the?greater the number of active sites?available and the?grea...
Rate: pH All enzymes have an?optimum pH?or a pH at which they operate best Enzymes are?denatured?at extremes of pH Hydrogen?and ionic bonds?hold the tertiary structure of the protein (ie. the enzym...
Rate: Temperature Enzymes have a?specific optimum temperature?– the temperature at which they catalyse a reaction at the?maximum rate Lower temperatures?either?prevent?reactions from proceeding or?...
Colorimetry to Measure Enzyme Activity A?colorimeter?is able to measure?light absorbance?(how much light is absorbed) or?light transmission?(how much light passes through) a substance Colorimetry c...
Measuring Enzyme Activity The?progress?of?enzyme-catalysed reactions?can be investigated by: Measuring the?rate of formation of a product?using catalase Measuring the?rate of disappearance of a sub...
