2019英特爾ISEF學科最佳作品摘要：生物化學 | Best of Category Abstract: Biochemistry

獲獎作品基本信息

年份	2019
學科	生物化學 Biochemistry
國家/州	CT，United States of America

獲獎作品名稱

Deuterium Oxide (D2O) on Maintaining Viability in Coliphage Bacteriophages under Low Temperatures to Model Live Attenuated Viral Vaccine Additives

獲獎作品摘要

Viral particles used in vaccines and viral therapies are often damaged due to the molecular movement of their storage solution and require storage at low temperatures to reduce the velocity of the water molecules. Deuterium oxide (D2O) is made with an isotope of hydrogen that increases the density of water to 1.11 g/mL; when viral particles are stored in D2O the increased weight reduces the molecular speed of the solution, reducing trauma to the particles, increasing the temperature in which samples can be stored. A T4 bacteriophage was used to test how a viral particle would react to its environment and deteriorate over time while stored in D2O and deionized water. A sample of Coliphage bacteriophages stored in D2O was compared to a sample stored in deionized water at 16℃ to determine the infectivity titer of the samples over time using plaque assays. The sample stored in D2O showed significantly less deterioration over time and slowed the rate of degradation to 6% that of deionized water. D2O proved to be a more advantageous solution than deionized water in supporting the health of the phages and is a promising storage additive for viral samples. This solution has application for use to increase the storage temperature of live attenuated viral vaccines, such as the Ebola vaccine, rVSV EBOV that often require storage at very low temperatures during transport and storage to remain effective and viable for administration to patients.

 

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ISEF 簡介

英特爾國際科學與工程大獎賽，簡稱 "ISEF"，由美國 Society for Science and the Public（科學和公共服務協會）主辦，英特爾公司冠名贊助，是全球規模最大、等級最高的中學生的科研科創賽事。ISEF 的學術活動學科包括了所有數學、自然科學、工程的全部領域和部分社會科學。ISEF 素有全球青少年科學學術活動的“世界杯”之美譽，旨在鼓勵學生團隊協作，開拓創新，長期專一深入地研究自己感興趣的課題。

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英特爾 ISEF 學術活動詳細介紹

英特爾 ISEF 全程指導方案

· 數學 · 物理 · 化學 · 生物 · 計算機 · 工程 ·

學科簡介：生物化學 Biochemistry

The study of the chemical basis of processes occurring in living organisms, including the processes by which these substances enter into, or are formed in, the organisms and react with each other and the environment.

Subcategories:

Analytical Biochemistry?(ANB):?The study of biochemical components found in a cell or other biological sample.?The study of the separation, identification, and quantification of chemical components relevant to living organisms.

General Biochemistry?(GNR):?The study of chemical and physiochemical?processes, including interactions and reactions, relevant to living organisms.

Medicinal Biochemistry?(MED):?The study of biochemical processes within the human body, with special reference to health and disease.

Structural Biochemistry?(STR):?The study of components, functions, and structures of molecules of cells within living organisms. Projects in this subcategory can address various variables of cells through the theories of Chemistry and the laws of Physics.

Other?(OTH):?Studies that cannot be assigned to one of the above subcategories. If the project involves multiple subcategories, the principal subcategory should be chosen instead of Other.