• The end product of glycolysis is?pyruvate
• Pyruvate contains a substantial amount of chemical energy that can be further utilised in respiration to produce more ATP
• When?oxygen is available?pyruvate will?enter the mitochondrial?matrix?and?aerobic?respiration will continue
• It moves across the double membrane of the mitochondria via?active transport
  • It requires a transport protein and a small amount of ATP
• Once in the mitochondrial matrix?pyruvate?takes part in the?link reaction

Pyruvate moving across the mitochondrial double membrane

• It is referred to as the link reaction because it?links?glycolysis?to the?Krebs cycle
• The steps are:

1.?Decarboxylation?and?dehydrogenation?of pyruvate by enzymes to produce an?acetyl group, CH3C(O)-

2.?Combination with coenzyme A?to form?acetyl CoA

• It produces:
  • Acetyl CoA
  • Carbon dioxide (CO2)
  • Reduced NAD (NADH)

pyruvate + NAD + CoA → acetyl CoA + carbon dioxide + reduced NAD

The link reaction

Role of coenzyme A

• A coenzyme is a molecule that?helps an enzyme carry out its function?but is?not used in the reaction itself
• Coenzyme A consists of a nucleoside (ribose and adenine) and a vitamin
• In the link reaction, CoA binds to the remainder of the pyruvate molecule (acetyl group 2C) to form acetyl CoA
• It then?supplies the acetyl group to the Krebs cycle?where it is used to continue aerobic respiration
• This is the stage that brings part of the carbohydrate (or lipid/amino acid) into the further stages of respiration and?links?the initial stage of respiration in the cytoplasm to the later stages in the mitochondria