• In the past scientists encountered many difficulties when trying to determine the evolutionary relationships of species
• Using the physical features of species (such as colour/shape/size) has many limitations and can often lead to the wrong classification of species
• Advances in genome sequencing and immunology has allowed scientists to further investigate the evolutionary relationships between species

Genome Sequencing

• Three types of sequence data are used to investigate evolutionary relationships
  • DNA
  • mRNA
  • Amino acids (of a protein)

   

• Sequencing technology can determine the order of DNA bases, mRNA bases and amino acids within an organism's genome
• This technology is especially useful for comparison with an extinct species (using ancient DNA) or when distinguishing between species that are very physically similar
• Scientists will choose?specific proteins or sections of the genome for comparison?between organisms
  • Looking at?multiple proteins or multiple regions of the genome?will allow for a more accurate estimate of evolutionary relatedness
  • Note the protein used needs to be present in a wide range of organisms and show sufficient variation between species
  • Cytochrome?c?is often used as it is an integral protein to respiration (in the electron transport chain) which is used by all eukaryotic organisms

   

• For all types of sequence data it can be said that the?more similar?the sequences, the?more closely related?the species are
• Two groups of organisms with very similar sequences will have?separated into separate species more recently?than two groups with less similarity in their sequences
• Species that have been separated for longer have had a greater amount of?time to accumulate mutations and changes?to their DNA,mRNA and amino acid sequences
• Sequence analysis and comparison can be used to create family trees that show the evolutionary relationships between species

The DNA base sequences of two closely related species being compared - Species Y is the ancestor of Species X.

Immunology

• The proteins of organisms can also be compared using immunological techniques
• The protein?albumin?is found in many species and is commonly used for these experiments
• Method:
  • Pure albumin samples are extracted from blood samples taken from multiple species
  • Each pure albumin sample is injected into a different rabbit
  • Each rabbit produces antibodies for that specific type of albumen
  • The different antibodies are extracted from the different rabbits and are then mixed with the different albumin samples
  • The precipitate (antibody-antigen complexes) resulting from each mixed sample is weighed

   

• Results and Interpretation:
  • The greater the weight of the precipitate, the greater the degree of complementarity between the antibody and albumin
  • For example, antibodies produced against human albumin will produce a larger amount of precipitate when exposed to chimpanzee albumin than when exposed to rat albumin because humans are more closely related to chimpanzees

   

An image showing the use of albumin and antibody production in comparing the relationship between different species