• The?greater?the?substrate concentration, the?higher?the?rate of reaction:
  • As the number of substrate molecules increases, the likelihood of enzyme-substrate complex formation increases
  • If the enzyme concentration remains?fixed?but the amount of substrate is increased past a certain point, however, all available active sites eventually become?saturated?and any further increase in substrate concentration will?not increase?the reaction rate
  • When the active sites of the enzymes are all full, any substrate molecules that are added have?nowhere to bind?in order to form an?enzyme-substrate complex
• For this reason, in the graph below there is a?linear increase?in reaction rate as substrate is added, which then?plateaus?when all active sites become occupied

The effect of substrate concentration on the rate of an enzyme-catalysed reaction