Drawing the ultrastructure of cells

• To record the observations seen under the microscope (or from photomicrographs taken) a labelled biological drawing is often made
• Biological drawings?are line pictures that show specific features that have been observed when the specimen was viewed
• There are a number of rules/conventions that are followed when making a biological drawing

Drawing conventions

• The drawing must have a title
• The?magnification?under which the observations shown by the drawing are made must be recorded
• A?sharp HB pencil?should be used (and a good eraser!)
• Drawings should be on plain white paper
• Lines should be?clear,?single?lines?(no thick shading)
• No shading
• The drawing should take up as much of the space on the page as possible
• Well-defined structures should be drawn
• The drawing should be made with?proper proportions
• Label lines?should not cross or have arrowheads and should?connect directly?to the part of the drawing being labelled
• Label lines should be kept to one side of the drawing (in parallel to the top of the page) and drawn with a?ruler
• Drawings of?cells?are typically made when visualizing cells at a?higher?magnification power, whereas?plan?drawings are typically made of tissues viewed under?lower?magnifications (individual cells are never drawn in a plan diagram)

• Due to the size of prokaryotes (0.1 to 5 μm) their ultrastructure can only be seen using an electron microscope
• Therefore drawings of prokaryotes are based on electron micrographs
• When viewing an electron micrograph of a prokaryote there is?no distinct dark circular area?within the cell, as there is?no nucleus?and?no organelles?are visible (apart from ribosomes, but as they are 70 S in size these are difficult to distinguish)

Transmission electron micrograph of a prokaryote and drawing

Scanning electron micrograph of an E.coli and drawing

• When viewing a eukaryotic cell under a light microscope it is possible to identify the nucleus and if it is a plant cell the cell wall and vacuole
• However, under an electron microscope, more detail of the ultrastructure of the eukaryotic cell can be seen
• The following organelles should be able to be identified, although it does depend on whether it is a plant or animal cell and the specialisation of the cell:
  • Rough endoplasmic reticulum
  • Golgi apparatus
  • Lysosomes
  • Vesicles
  • Ribosomes
  • Vacuole (plant)
  • Nucleus
  • Mitochondrion
  • Chloroplast
• The nucleus, mitochondrion and chloroplast all have double membranes
• The cell wall will be present in plant eukaryotic cells. This is an extra-cellular component

TEM electron micrograph of an animal cell showing key features

TEM electron micrograph of a plant cell showing key features

• Electron microscopes can produce highly detailed images of animal and plant cells
• The key cellular structures within animal and plant cells are visible within the electron micrographs above
  • The presence of a vacuole in a micrograph is a good indicator of the cell type

Electron micrograph of the nucleus

• The nucleus should be clearly identifiable as it is the largest structure in the eukaryotic cell

Electron micrograph of the mitochondrion

• To identify the mitochondrion look for the?crista (the foldings of the inner membrane) which are often visible in electron micrographs

Electron micrograph of the rough endoplasmic reticulum

• The rough endoplasmic reticulum (rER) is located next to the nucleus and the attached ribosomes can be used to identify the rER as they make the membrane appear darker

Electron micrograph of the chloroplast

• The chloroplast can be identified by the?thylakoid stacks?(grana), as they appear as dark lines within the organelle
• Chloroplasts are large

Electron micrograph of the Golgi apparatus

• Golgi apparatus will be located near the endoplasmic reticulum and it:
  • Does not have long membrane sacs
  • The sacs are more curved than the endoplasmic reticulum
  • Does not have ribosomes attached
  • Has many vesicles close by

Electron micrograph of the vesicles

• Vesicles are spherical shapes

Electron micrograph of the ribosomes

• Free ribosomes appear as dark granules (tiny dark dots) in the cytoplasm

Electron micrograph and drawing of a palisade mesophyll cell

• The palisade mesophyll electron micrograph will have:
  • The?chloroplasts?along the plasma membrane as this is where the most light can be absorbed
  • A large?vacuole?in the centre
  • A cell wall

Electron micrograph and drawing of an exocrine gland cell of the pancreas

• An exocrine gland cell of the pancreas will:
  • Have many large secretory vesicles (carrying the digestive enzymes)
  • Have many?mitochondria
  • Be densely packed with rough endoplasmic reticulum