• Enzymes are?globular proteins
• This means their?3D?shape?(as well as the shape of the?active site?of an enzyme) is determined by the complex tertiary structure of the protein that makes up the enzyme and is therefore?highly specific
• Enzymes have a unique?active site?where?specific substrates bind?forming an?enzyme-substrate complex
• The?active site?of an enzyme has a?specific shape?to fit a?specific substrate
• Extremes of?heat?or?pH?can alter the protein structure and?change the shape?of the active site,?preventing?substrate binding – this is called?denaturation
• Substrates?collide?with the enzymes active site and this must happen at the?correct orientation and speed?in order for a reaction to occur

The active site of an enzyme has a specific shape to fit a specific substrate (when the substrate binds an enzyme-substrate complex is formed)

Enzyme specificity

• The?specificity?of an enzyme is a result of the?complementary nature?between the?shape?of the?active site?on the enzyme and its?substrate(s)
• Only?one specific substrate will fit into one specific active site
• The shape of the active site (and therefore the specificity of the enzyme) is determined by the?complex tertiary structure?of the?protein?that makes up the enzyme:
  • Proteins are formed from chains of amino acids held together by peptide bonds
  • The order of amino acids determines the shape of an enzyme
  • If the order is altered, the resulting three-dimensional shape changes
• If the?tertiary structure of the protein is altered?in any way, the shape of the?active site will change?and the substrate will no longer fit the active site
• This means that an enzyme-substrate complex will not be able to form and the product(s) will not be produced:?the enzyme will not be able to carry out its function

An example of enzyme specificity

The lock-and-key hypothesis

• In the 1890’s the first model of enzyme activity was described by Emil Fischer:
  • He suggested that both enzymes and substrates were?rigid structures?that?locked?into each other very?precisely, much like a key going into a lock
  • This is known as the ‘lock-and-key hypothesis’

The Lock and Key hypothesis

The induced-fit hypothesis

• The lock-and-key model was later?modified?and adapted to our current understanding of enzyme activity, permitted by advances in techniques in the molecular sciences
• The?modified?model?of enzyme activity (first proposed in 1959) is known as the ‘induced-fit hypothesis’
• Although it is very similar to the lock and key hypothesis, in this model the enzyme and substrate?interact?with each other:
  • The enzyme and its active site (and sometimes the substrate) can?change shape?slightly as the substrate molecule enters the enzyme
  • These changes in shape are known as?conformational changes
  • The conformational changes ensure an?ideal binding arrangement?between the enzyme and substrate is achieved
  • This?maximises the ability of the enzyme to catalyse the reaction

The Induced Fit model of enzyme action