2019英特爾ISEF學科最佳作品摘要：微生物學 Best of Category Abstract: MICROBIOLOGY

獲獎作品基本信息

年份	2019
學科	微生物學 MICROBIOLOGY
國家/州	NY，United States of America

獲獎作品名稱

Investigating the Role of the Novel ESCRT-III Recruiter CCDC11 in HIV Viral Budding: Identifying a Potential Target for Antiviral Therapy

獲獎作品摘要

Acquired immunodeficiency syndrome (AIDS) is a life-threatening condition caused by infection of the human immunodeficiency virus (HIV). Compromising the immune system, HIV reduces the body's ability to fight the organisms that cause disease. In an effort to discover potential targets for antiviral therapies, we explored the role of coiled-coil domain-containing 11 (CCDC11) in HIV-1 budding. CCDC11 plays an important role in ciliogenesis and cytokinesis. During cytokinesis, it recruits the endosomal sorting complex required for transport III (ESCRT-III) membrane scission complex to the midbody to mediate the physical separation of two dividing daughter cells. The ESCRT-III machinery is also integral to the viral budding process of HIV-1 and many other viruses. Therefore, we hypothesized that CCDC11 is also required for viral budding. To investigate this possibility, we established CCDC11-knockout human embryonic kidney (HEK) 293T cells using the CRISPR-Cas9 technology. Indels of the CCDC11 gene were confirmed by DNA sequencing, and protein levels were assessed via western blotting and immunofluorescence staining. To determine the effect of CCDC11-knockout on HIV-1 budding, we employed the Enzyme-linked immunosorbent assay (ELISA) p24 capture assay to indirectly assess viral release by measuring the relative concentration of HIV-1 Gag structural protein (p24) or mutant P7L-Gag in cell media. Our results demonstrate that ectopic overexpression of CCDC11 markedly enhances whereas depletion of CCDC11 in the knockout cells dramatically reduces viral particle release. The defective viral budding in CCDC11-knockout cells was restored when wild-type CCDC11 was re-expressed. Collectively, our data suggest that CCDC11 is critical for efficient HIV-1 budding.

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高中生科研學術活動 英特爾 Intel ISEF 簡介

英特爾國際科學與工程大獎賽，簡稱 "ISEF"，由美國 Society for Science and the Public（科學和公共服務協會）主辦，英特爾公司冠名贊助，是全球規模最大、等級最高的中學生的科研科創賽事。ISEF 的學術活動學科包括了所有數學、自然科學、工程的全部領域和部分社會科學。ISEF 素有全球青少年科學學術活動的“世界杯”之美譽，旨在鼓勵學生團隊協作，開拓創新，長期專一深入地研究自己感興趣的課題。

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英特爾 ISEF 學術活動詳細介紹

英特爾 ISEF 全程指導方案

· 數學 · 物理 · 化學 · 生物 · 計算機 · 工程 ·

學科簡介：微生物學 MICROBIOLOGY

The study of micro-organisms, including bacteria, viruses, fungi, prokaryotes, and simple eukaryotes as well as antimicrobial and antibiotic substances.

Subcategories:

Antimicrobials and Antibiotics?(ANT):?Studies involving substances that kill or inhibit the growth of a microorganism.

Applied Microbiology?(APL):?The study of microorganisms having potential applications in human, animal or plant health or the use of microorganisms in the production of energy.

Bacteriology?(BAC):?The study of bacteria and bacterial diseases and the microorganisms responsible for causing a disease.?This field focuses on ideas spanning a wide range of topics, from identification and characterization of bacteria, all the way to the development of effective vacancies to combat various types of bacteria.

Environmental Microbiology?(ENV):?Studies involving microbial interactions and processes within the environment.? Air microbiology, soil microbiology and water microbiology as well as the study of biofilms would be included in this subcategory.

Microbial Genetics?(GEN):?The study of the genetics of microorganisms such as bacteria, archaea and some protozoa and fungi and their chromosomes, plasmids, transposons and phages.?? Studies can also include gene transfer systems such as transformation, conjugation and transduction.

Virology?(VIR):?The study of viruses – submicroscopic, parasitic particles of genetic material contained in a protein coat – and virus-like agents. Research in this subcategory may focus on the development and effectiveness of treatments for viruses, the development and life cycle of a particular virus, or how the immune system recognizes a virus and what stimulates immune responses.

Other?(OTH):?Studies that cannot be assigned to one of the above subcategories. If the project involves multiple subcategories, the principal subcategory should be chosen instead of Other.