2018英特爾ISEF學科最佳作品摘要：植物科學 Best of Category Abstract: PLANT SCIENCES

獲獎作品基本信息

年份	2018
學科	植物科學 PLANT SCIENCES
學校	No. 2 High School of East China Normal University
國家/州	China

獲獎作品名稱

Crude Extraction of Amana edulis Induces Liver Cancer Apoptosis

獲獎作品摘要

HCC has become the fastest-rising cause of cancer-related death. A novel therapeutic approach for treatment is warranted. The goal of this study is to find effective components from Chinese herbal medicines, which is an important alternative source of anti-cancer medicines. To this end, six different herbs were selected from various traditional literatures. Then, Soxhlet extractor was used to distill the strong polar and weak polar components of each herb. The inhibitive effect of each component was determined using liver cancer cells BEL7404. From total of 12 extractions, it was found that the combined crude lysate of Amana Edulis from water and ethanol system had the best efficacy. At the concentration of 0.1 mg/mL, this component has a highest inhibition rate up to 70%. To investigate the potential molecular reasons, we observed that the component can significantly induce the liver cancer cells apoptosis and retard the cell reproduction at G2/M stage. Verification experiments showed that this component also has apparent inhibitive effects on other liver cancer cells, such as HepG2 and Huh7. On the other hand, it has less effectiveness on another cell line HepaRG, which retains many characteristics of primary human hepatocytes. The results suggested that there might be highly efficient anti-hepatoma ingredient in the water and ethanol extraction of Amana Edulis. The pure substances remain to be isolated and further research on their targets are required.

Keywords: Chinese herbal medicine; Amana edulis; Apoptosis; Liver cancer

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高中生科研學術活動 英特爾 Intel ISEF 簡介

英特爾國際科學與工程大獎賽，簡稱 "ISEF"，由美國 Society for Science and the Public（科學和公共服務協會）主辦，英特爾公司冠名贊助，是全球規模最大、等級最高的中學生的科研科創賽事。ISEF 的學術活動學科包括了所有數學、自然科學、工程的全部領域和部分社會科學。ISEF 素有全球青少年科學學術活動的“世界杯”之美譽，旨在鼓勵學生團隊協作，開拓創新，長期專一深入地研究自己感興趣的課題。

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英特爾 ISEF 學術活動詳細介紹

英特爾 ISEF 全程指導方案

· 數學 · 物理 · 化學 · 生物 · 計算機 · 工程 ·

學科簡介：植物科學 PLANT SCIENCES

Studies of plants and how they live, including structure, physiology, development, and classification. Includes plant cultivation, development, ecology, genetics and plant breeding, pathology, physiology, systematics and evolution.

Subcategories:

Agriculture and Agronomy?(AGR):?Application of the various soil and plant sciences to soil management and agricultural and horticultural crop production. Includes biological and chemical controls of pests, hydroponics, fertilizers and supplements.

Ecology?(ECO):?The study of interactions and relationships among plants, and plants and animals, with their environment.

Genetics and Breeding?(GEN):?The study of organismic and population genetics of plants. The application of plant genetics and biotechnology to crop improvement.?This includes genetically modified crops.

Growth and Development?(DEV):?The study of a plant from earliest stages through germination and into later life. This includes cellular and molecular aspects of development and environmental effects, natural or manmade, on development and growth.

Pathology?(PAT):?The study of plant disease states, and their causes, processes, and consequences. This includes effects of parasites or disease-causing microbes.

Plant Physiology?(PHY):?The study of functions in plants and plant cells. This includes cellular mechanisms such as photosynthesis and transpiration, and how plant processes are affected by environmental factors or natural variations.

Systematics and Evolution?(SYS):?The study of classification of organisms and their evolutionary relationships. This includes morphological, biochemical, genetic, and modeled systems.

Other?(OTH):?Studies that cannot be assigned to one of the above subcategories. If the project involves multiple subcategories, the principal subcategory should be chosen instead of Other.